Latest publications of LIFS consortium members
Latest publications of LIFS consortium members: Latest results from PubMed-
Glycerophospholipid remodeling is critical for orthoflavivirus infection
07 October 2024
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Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards
03 October 2024
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The lipidomics reporting checklist a framework for transparency of lipidomic experiments and repurposing resource data
16 August 2024
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Pitfalls in lipid mass spectrometry of mammalian samples - a brief guide for biologists
01 July 2024
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Communicating Mass Spectrometry Quality Information in mzQC with Python, R, and Java
26 June 2024
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A Targeted, Bioinert LC-MS/MS Method for Sensitive, Comprehensive Analysis of Signaling Lipids
25 May 2024
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Lipidome Unsaturation Affects the Morphology and Proteome of the Drosophila Eye
14 March 2024
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Challenges and perspectives for naming lipids in the context of lipidomics
24 January 2024
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Multispecies Benchmark Analysis for LC-MS/MS Validation and Performance Evaluation in Bottom-Up Proteomics
20 January 2024
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Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
11 December 2023
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Eye proteome of Drosophila melanogaster
14 November 2023
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LipidSpace: Simple Exploration, Reanalysis, and Quality Control of Large-Scale Lipidomics Studies
04 October 2023
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LORA, Lipid Over-Representation Analysis Based on Structural Information
16 August 2023
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Absolute Quantification of Photoreceptor Outer Segment Proteins
26 July 2023
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Global analysis of putative phospholipases in Plasmodium falciparum reveals an essential role of the phosphoinositide-specific phospholipase C in parasite maturation
25 July 2023
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Lipidome unsaturation affects the morphology and proteome of the Drosophila eye
22 May 2023
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Ex vivo instability of lipids in whole blood: preanalytical recommendations for clinical lipidomics studies
22 April 2023
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Quantification of bulk lipid species in human platelets and their thrombin-induced release
15 April 2023
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Eye proteome of Drosophila melanogaster
22 March 2023
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Pepstatin-Based Probes for Photoaffinity Labeling of Aspartic Proteases and Application to Target Identification
15 March 2023
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Proteomics Standards Initiative at Twenty Years: Current Activities and Future Work
10 January 2023
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Guiding the choice of informatics software and tools for lipidomics research applications
21 December 2022
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Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro
29 November 2022
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Ecological lipidology
07 September 2022
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Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
26 August 2022
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Introducing the Lipidomics Minimal Reporting Checklist
07 August 2022
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Platelet lipid metabolism in vascular thrombo-inflammation
31 July 2022
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A Current Encyclopedia of Bioinformatics Tools, Data Formats and Resources for Mass Spectrometry Lipidomics
27 July 2022
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Sortase A-Cleavable CD1d Identifies Sphingomyelins as Major Class of CD1d-Associated Lipids
25 July 2022
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Are n-3 PUFAs from Microalgae Incorporated into Membrane and Storage Lipids in Pig Muscle Tissues?-A Lipidomic Approach
25 July 2022
Introducing the Lipidomics Minimal Reporting Checklist
Abstract
The rapid increase in lipidomic data has triggered a community-based movement to develop guidelines and minimum requirements for generating, reporting and publishing lipidomic data. The creation of a dynamic checklist summarizing key details of lipidomic analyses using a common language has the potential to harmonize the field by improving both traceability and reproducibility.
McDonald, J. G. et al., Introducing the Lipidomics Minimal Reporting Checklist, Nature Metabolism (2022)
doi: 10.1038/s42255-022-00628-3Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures
Abstract
A strong inflammatory immune response drives the lung pathology in neonatal acute respiratory distress syndrome (nARDS). Anti-inflammatory therapy is therefore a promising strategy for improved treatment of nARDS. We demonstrate a new function of the anionic phospholipids POPG, DOPG, and PIP2 as inhibitors of IL-1β release by LPS and ATP-induced inflammasome activation in human monocyte-derived and lung macrophages. Curosurf® surfactant was enriched with POPG, DOPG, PIP2 and the head-group derivative IP3, biophysically characterized and applicability was evaluated in a piglet model of nARDS. The composition of pulmonary surfactant from piglets was determined by shotgun lipidomics screens. After 72 h of nARDS, levels of POPG, DOPG, and PIP2 were enhanced in the respective treatment groups. Otherwise, we did not observe changes of individual lipid species in any of the groups. Surfactant proteins were not affected, with the exception of the IP3 treated group. Our data show that POPG, DOPG, and PIP2 are potent inhibitors of inflammasome activation; their enrichment in a surfactant preparation did not induce any negative effects on lipid profile and reduced biophysical function in vitro was mainly observed for PIP2. These results encourage to rethink the current strategies of improving surfactant preparations by inclusion of anionic lipids as potent anti-inflammatory immune regulators.
Kupsch, S. et al., Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures, European Journal of Pharmaceutical Sciences (2022)
doi:10.1016/j.ejps.2022.106216Are n-3 PUFAs from Microalgae Incorporated into Membrane and Storage Lipids in Pig Muscle Tissues?-A Lipidomic Approach
Abstract
One-fourth of the global human population is estimated to be infected with strains of the Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB). Using lipidomic approaches, we show that tuberculostearic acid (TSA)-containing phosphatidylinositols (PIs) are molecular markers for infection with clinically relevant MTBC strains and signify bacterial burden. For the most abundant lipid marker, detection limits of ∼102 colony forming units (CFUs) and ∼103 CFUs for bacterial and cell culture systems were determined, respectively. We developed a targeted lipid assay, which can be performed within a day including sample preparation─roughly 30-fold faster than in conventional methods based on bacterial culture. This indirect and culture-free detection approach allowed us to determine pathogen loads in infected murine macrophages, human neutrophils, and murine lung tissue. These marker lipids inferred from mycobacterial PIs were found in higher levels in peripheral blood mononuclear cells of TB patients compared to healthy individuals. Moreover, in a small cohort of drug-susceptible TB patients, elevated levels of these molecular markers were detected at the start of therapy and declined upon successful anti-TB treatment. Thus, the concentration of TSA-containing PIs can be used as a correlate for the mycobacterial burden in experimental models and in vitro systems and may prospectively also provide a clinically relevant tool to monitor TB severity.
Dannenberger, D. et al., Tuberculostearic Acid-Containing Phosphatidylinositols as Markers of Bacterial Burden in Tuberculosis, ACS Omega (2022)
doi:10.1021/acsomega.2c02476- Tuberculostearic Acid-Containing Phosphatidylinositols as Markers of Bacterial Burden in Tuberculosis
- A Current Encyclopedia of Bioinformatics Tools, Data Formats and Resources for Mass Spectrometry Lipidomics
- Goslin 2.0
- Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment