LIFS Tools
  • Home
  • About
  • News
  • Training
  • Publications
  • Service & Support
  • Tools
    • Goslin
    • LipidCreator
    • LipidXplorer
    • LxPostman
    • LipidSpace
    • Lipidome Projector
    • LipidCompass
    • LUX Score
  •  
LIFS Tools

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards

Abstract

In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). 

Torta, F. T., Hoffmann, N., Burla, B. et al., Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards, Nature Communications (2024)

doi: 10.1038/s41467-024-52087-x

 

PUBMed Publication Feed

Latest publications of LIFS consortium members

Latest publications of LIFS consortium members: Latest results from PubMed
  • Introduction of a Lipidomics Scoring System for data quality assessment
    02 May 2025
  • Lipidome visualisation, comparison, and analysis in a vector space
    15 April 2025
  • A reference database enabling in-depth proteome and PTM analysis of mouse immune cells
    10 April 2025
  • Targeted Absolute Quantification of Protein Biomarkers in Cerebrospinal Fluid by FastCAT
    01 April 2025
  • Dengue virus is particularly sensitive to interference with long-chain fatty acid elongation and desaturation
    25 January 2025
  • Synaptoneurolipidomics: lipidomics in the study of synaptic function
    03 January 2025
  • The Proteomics Standards Initiative Standardized Formats for Spectral Libraries and Fragment Ion Peak Annotations: mzSpecLib and mzPAF
    08 November 2024
  • Glycerophospholipid remodeling is critical for orthoflavivirus infection
    07 October 2024
  • Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards
    03 October 2024
  • The lipidomics reporting checklist a framework for transparency of lipidomic experiments and repurposing resource data
    16 August 2024
  • Pitfalls in lipid mass spectrometry of mammalian samples - a brief guide for biologists
    01 July 2024
  • Communicating Mass Spectrometry Quality Information in mzQC with Python, R, and Java
    26 June 2024
  • A Targeted, Bioinert LC-MS/MS Method for Sensitive, Comprehensive Analysis of Signaling Lipids
    25 May 2024
  • Lipidome Unsaturation Affects the Morphology and Proteome of the Drosophila Eye
    14 March 2024
  • Challenges and perspectives for naming lipids in the context of lipidomics
    24 January 2024
  • Multispecies Benchmark Analysis for LC-MS/MS Validation and Performance Evaluation in Bottom-Up Proteomics
    20 January 2024
  • Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
    11 December 2023
  • Eye proteome of Drosophila melanogaster
    14 November 2023
  • LipidSpace: Simple Exploration, Reanalysis, and Quality Control of Large-Scale Lipidomics Studies
    04 October 2023
  • LORA, Lipid Over-Representation Analysis Based on Structural Information
    16 August 2023
  • Absolute Quantification of Photoreceptor Outer Segment Proteins
    26 July 2023
  • Global analysis of putative phospholipases in Plasmodium falciparum reveals an essential role of the phosphoinositide-specific phospholipase C in parasite maturation
    25 July 2023
  • Lipidome unsaturation affects the morphology and proteome of the Drosophila eye
    22 May 2023
  • Ex vivo instability of lipids in whole blood: preanalytical recommendations for clinical lipidomics studies
    22 April 2023
  • Quantification of bulk lipid species in human platelets and their thrombin-induced release
    15 April 2023
  • Eye proteome of Drosophila melanogaster
    22 March 2023
  • Pepstatin-Based Probes for Photoaffinity Labeling of Aspartic Proteases and Application to Target Identification
    15 March 2023
  • Proteomics Standards Initiative at Twenty Years: Current Activities and Future Work
    10 January 2023
  • Guiding the choice of informatics software and tools for lipidomics research applications
    21 December 2022
  • Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro
    29 November 2022

Introducing the Lipidomics Minimal Reporting Checklist

Introducing the Lipidomics Minimal Reporting Checklist

Abstract

The rapid increase in lipidomic data has triggered a community-based movement to develop guidelines and minimum requirements for generating, reporting and publishing lipidomic data. The creation of a dynamic checklist summarizing key details of lipidomic analyses using a common language has the potential to harmonize the field by improving both traceability and reproducibility.

McDonald, J. G. et al., Introducing the Lipidomics Minimal Reporting Checklist, Nature Metabolism (2022)

doi: 10.1038/s42255-022-00628-3

Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures

Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures

Abstract

A strong inflammatory immune response drives the lung pathology in neonatal acute respiratory distress syndrome (nARDS). Anti-inflammatory therapy is therefore a promising strategy for improved treatment of nARDS. We demonstrate a new function of the anionic phospholipids POPG, DOPG, and PIP2 as inhibitors of IL-1β release by LPS and ATP-induced inflammasome activation in human monocyte-derived and lung macrophages. Curosurf® surfactant was enriched with POPG, DOPG, PIP2 and the head-group derivative IP3, biophysically characterized and applicability was evaluated in a piglet model of nARDS. The composition of pulmonary surfactant from piglets was determined by shotgun lipidomics screens. After 72 h of nARDS, levels of POPG, DOPG, and PIP2 were enhanced in the respective treatment groups. Otherwise, we did not observe changes of individual lipid species in any of the groups. Surfactant proteins were not affected, with the exception of the IP3 treated group. Our data show that POPG, DOPG, and PIP2 are potent inhibitors of inflammasome activation; their enrichment in a surfactant preparation did not induce any negative effects on lipid profile and reduced biophysical function in vitro was mainly observed for PIP2. These results encourage to rethink the current strategies of improving surfactant preparations by inclusion of anionic lipids as potent anti-inflammatory immune regulators.

Kupsch, S. et al., Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures, European Journal of Pharmaceutical Sciences (2022)

doi:10.1016/j.ejps.2022.106216

More Articles …

  1. Are n-3 PUFAs from Microalgae Incorporated into Membrane and Storage Lipids in Pig Muscle Tissues?-A Lipidomic Approach
  2. Tuberculostearic Acid-Containing Phosphatidylinositols as Markers of Bacterial Burden in Tuberculosis
  3. A Current Encyclopedia of Bioinformatics Tools, Data Formats and Resources for Mass Spectrometry Lipidomics
  4. Goslin 2.0

Page 1 of 7

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7

Uni Wien
l.mpi
fz borstel
Forschungszentrum Jülich

 
Supported by the German BMBF
Supported by the Austrian BMBWF
  • Imprint & Privacy Policy
  • Impressum & Datenschutzerklärung
  • Terms of Service
  • Funding
© 2016 - 2025 LIFS Tools Consortium
Top
  • Home
  • About
  • News
  • Training
  • Publications
  • Service & Support
  • Tools
    • Goslin
    • LipidCreator
    • LipidXplorer
    • LxPostman
    • LipidSpace
    • Lipidome Projector
    • LipidCompass
    • LUX Score
  •  
  • Imprint & Privacy Policy
  • Impressum & Datenschutzerklärung
  • Terms of Service
  • Funding