Latest publications of LIFS consortium members
Latest publications of LIFS consortium members: Latest results from PubMed- Eye proteome of Drosophila melanogaster
- LipidSpace: Simple Exploration, Reanalysis, and Quality Control of Large-Scale Lipidomics Studies
- LORA, Lipid Over-Representation Analysis Based on Structural Information
- Absolute Quantification of Photoreceptor Outer Segment Proteins
- Global analysis of putative phospholipases in Plasmodium falciparum reveals an essential role of the phosphoinositide-specific phospholipase C in parasite maturation
- Lipidome unsaturation affects the morphology and proteome of the Drosophila eye
- Ex vivo instability of lipids in whole blood: preanalytical recommendations for clinical lipidomics studies
- Quantification of bulk lipid species in human platelets and their thrombin-induced release
- Eye proteome of Drosophila melanogaster
- Pepstatin-Based Probes for Photoaffinity Labeling of Aspartic Proteases and Application to Target Identification
- Proteomics Standards Initiative at Twenty Years: Current Activities and Future Work
- Guiding the choice of informatics software and tools for lipidomics research applications
- Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro
- Ecological lipidology
- Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome
- Introducing the Lipidomics Minimal Reporting Checklist
- Platelet lipid metabolism in vascular thrombo-inflammation
- A Current Encyclopedia of Bioinformatics Tools, Data Formats and Resources for Mass Spectrometry Lipidomics
- Sortase A-Cleavable CD1d Identifies Sphingomyelins as Major Class of CD1d-Associated Lipids
- Are n-3 PUFAs from Microalgae Incorporated into Membrane and Storage Lipids in Pig Muscle Tissues?-A Lipidomic Approach
- Distinct glycerophospholipids potentiate Gsα-activated adenylyl cyclase activity
- Tuberculostearic Acid-Containing Phosphatidylinositols as Markers of Bacterial Burden in Tuberculosis
- The PNPLA3 variant I148M reveals protective effects toward hepatocellular carcinoma in mice via restoration of omega-3 polyunsaturated fats
- Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures
- FastCAT Accelerates Absolute Quantification of Proteins Using Multiple Short Nonpurified Chimeric Standards
- Goslin 2.0 Implements the Recent Lipid Shorthand Nomenclature for MS-Derived Lipid Structures
- Shedding light on silica biomineralization by comparative analysis of the silica-associated proteomes from three diatom species
- Toward Zero Variance in Proteomics Sample Preparation: Positive-Pressure FASP in 96-Well Format (PF96) Enables Highly Reproducible, Time- and Cost-Efficient Analysis of Sample Cohorts
- The human LL-37 peptide exerts antimicrobial activity against Legionella micdadei interacting with membrane phospholipids
- Remodeling of Lipid A in Pseudomonas syringae pv. phaseolicola In Vitro
- Lipidomic profiling of human serum enables detection of pancreatic cancer
- Analytical comparison of absolute quantification strategies to investigate the insulin signaling pathway in fat cells
- Median-Based Absolute Quantification of Proteins Using Fully Unlabeled Generic Internal Standard (FUGIS)
- Intracellular Lipid Accumulation and Mitochondrial Dysfunction Accompanies Endoplasmic Reticulum Stress Caused by Loss of the Co-chaperone DNAJC3
- Recommendations for good practice in MS-based lipidomics
- Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment
- Targeting early stages of cardiotoxicity from anti-PD1 immune checkpoint inhibitor therapy
- Nonalcoholic fatty liver disease stratification by liver lipidomics
- Quality control requirements for the correct annotation of lipidomics data
- LAMP3 deficiency affects surfactant homeostasis in mice
- The Colorectal Cancer Lipidome: Identification of a Robust Tumor-Specific Lipid Species Signature
- Targeted Phosphoinositides Analysis Using High-Performance Ion Chromatography-Coupled Selected Reaction Monitoring Mass Spectrometry
- Shotgun lipidomics and mass spectrometry imaging unveil diversity and dynamics in Gammarus fossarum lipid composition
- Mild hyperlipidemia in mice aggravates platelet responsiveness in thrombus formation and exploration of platelet proteome and lipidome
- Uncovering the complexity of the yeast lipidome by means of nLC/NSI-MS/MS
- Hydroxylated sphingolipid biosynthesis regulates photoreceptor apical domain morphogenesis
- Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics
- Lipidomics needs more standardization
- Absolute Quantification of Proteins in the Eye of Drosophila melanogaster
- Loss of hepatic Mboat7 leads to liver fibrosis
Introducing the Lipidomics Minimal Reporting Checklist
Abstract
The rapid increase in lipidomic data has triggered a community-based movement to develop guidelines and minimum requirements for generating, reporting and publishing lipidomic data. The creation of a dynamic checklist summarizing key details of lipidomic analyses using a common language has the potential to harmonize the field by improving both traceability and reproducibility.
McDonald, J. G. et al., Introducing the Lipidomics Minimal Reporting Checklist, Nature Metabolism (2022)
doi: 10.1038/s42255-022-00628-3Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures
Abstract
A strong inflammatory immune response drives the lung pathology in neonatal acute respiratory distress syndrome (nARDS). Anti-inflammatory therapy is therefore a promising strategy for improved treatment of nARDS. We demonstrate a new function of the anionic phospholipids POPG, DOPG, and PIP2 as inhibitors of IL-1β release by LPS and ATP-induced inflammasome activation in human monocyte-derived and lung macrophages. Curosurf® surfactant was enriched with POPG, DOPG, PIP2 and the head-group derivative IP3, biophysically characterized and applicability was evaluated in a piglet model of nARDS. The composition of pulmonary surfactant from piglets was determined by shotgun lipidomics screens. After 72 h of nARDS, levels of POPG, DOPG, and PIP2 were enhanced in the respective treatment groups. Otherwise, we did not observe changes of individual lipid species in any of the groups. Surfactant proteins were not affected, with the exception of the IP3 treated group. Our data show that POPG, DOPG, and PIP2 are potent inhibitors of inflammasome activation; their enrichment in a surfactant preparation did not induce any negative effects on lipid profile and reduced biophysical function in vitro was mainly observed for PIP2. These results encourage to rethink the current strategies of improving surfactant preparations by inclusion of anionic lipids as potent anti-inflammatory immune regulators.
Kupsch, S. et al., Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures, European Journal of Pharmaceutical Sciences (2022)
doi:10.1016/j.ejps.2022.106216Are n-3 PUFAs from Microalgae Incorporated into Membrane and Storage Lipids in Pig Muscle Tissues?-A Lipidomic Approach
Abstract
One-fourth of the global human population is estimated to be infected with strains of the Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB). Using lipidomic approaches, we show that tuberculostearic acid (TSA)-containing phosphatidylinositols (PIs) are molecular markers for infection with clinically relevant MTBC strains and signify bacterial burden. For the most abundant lipid marker, detection limits of ∼102 colony forming units (CFUs) and ∼103 CFUs for bacterial and cell culture systems were determined, respectively. We developed a targeted lipid assay, which can be performed within a day including sample preparation─roughly 30-fold faster than in conventional methods based on bacterial culture. This indirect and culture-free detection approach allowed us to determine pathogen loads in infected murine macrophages, human neutrophils, and murine lung tissue. These marker lipids inferred from mycobacterial PIs were found in higher levels in peripheral blood mononuclear cells of TB patients compared to healthy individuals. Moreover, in a small cohort of drug-susceptible TB patients, elevated levels of these molecular markers were detected at the start of therapy and declined upon successful anti-TB treatment. Thus, the concentration of TSA-containing PIs can be used as a correlate for the mycobacterial burden in experimental models and in vitro systems and may prospectively also provide a clinically relevant tool to monitor TB severity.
Dannenberger, D. et al., Tuberculostearic Acid-Containing Phosphatidylinositols as Markers of Bacterial Burden in Tuberculosis, ACS Omega (2022)
doi:10.1021/acsomega.2c02476- Tuberculostearic Acid-Containing Phosphatidylinositols as Markers of Bacterial Burden in Tuberculosis
- A Current Encyclopedia of Bioinformatics Tools, Data Formats and Resources for Mass Spectrometry Lipidomics
- Goslin 2.0
- Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment
