Shotgun Lipidomics Approach for Clinical Samples
Abstract
Shotgun lipidomics offers fast and reproducible identification and quantification of lipids in clinical samples. Lipid extraction procedures based on the methyl tert-butyl protocol are well established for performing shotgun lipidomics in biomedical research. Here, we describe a shotgun lipidomics workflow that is well suited for the analysis of clinical samples such as tissue samples, blood plasma, and peripheral blood mononuclear cells.
Eggers, L. F. & Schwudke, D. Shotgun Lipidomics Approach for Clinical Samples. in Clinical Metabolomics: Methods and Protocols (ed. Giera, M.) 163–174 (Springer New York, 2018). doi:10.1007/978-1-4939-7592-1_12
