Lysosome-associated membrane glycoprotein 3 (LAMP3) is a type I transmembrane protein of the LAMP protein family with a cell-type-specific expression in alveolar type II cells in mice and hitherto unknown function. In type II pneumocytes, LAMP3 is localized in lamellar bodies, secretory organelles releasing pulmonary surfactant into the extracellular space to lower surface tension at the air/liquid interface. The physiological function of LAMP3, however, remains enigmatic. We generated Lamp3 knockout mice by CRISPR/Cas9. LAMP3 deficient mice are viable with an average life span and display regular lung function under basal conditions. The levels of a major hydrophobic protein component of pulmonary surfactant, SP-C, are strongly increased in the lung of Lamp3 knockout mice, and the lipid composition of the bronchoalveolar lavage shows mild but significant changes, resulting in alterations in surfactant functionality. In ovalbumin-induced experimental allergic asthma, the changes in lipid composition are aggravated, and LAMP3-deficient mice exert an increased airway resistance. Our data suggest a critical role of LAMP3 in the regulation of pulmonary surfactant homeostasis and normal lung function.
This shotgun lipidomics data set includes the raw unimputed lipidomics values for lung tissue and bronchoalveolar fluid in mol percentage as well as on a per protein content base. Data can be found in the LAMP3_LipidomicsRawData.xlsx file.
Description and details of sample extraction to data processing procedures are included in the LAMP3_Processing.xlsx file. Additional project files for LipidXplorer and the complementary MFQL files are included in the corresponding folders.
Lars P. Lunding, Daniel Krause, Guido Stichtenoth, Cordula Stamme, Niklas Lauterbach, Jan Hegermann, Matthias Ochs, Björn Schuster, Radislav Sedlacek, Paul Saftig, Dominik Schwudke, Michael Wegmann, Markus Damme. LAMP3 is critical for surfactant homeostasis in mice, doi:10.1101/2021.02.05.429758
Preliminary LipidCompass accession number: LCE7