Educators:
Dominik Kopczynski (Uni Vienna, LIFS), Nils Hoffmann (FZ Jülich, LIFS)
Location:
On-Site training.
Contents:
In the first part of the course, we will work through an example for
targeted LC-MS lipidomics with LipidCreator and Skyline. In the second
part of the course, we will apply PeakStrainer and LipidXplorer for
filtering, identification and quantification of lipid signals from
shotgun lipidomics. The course will consist of a short theory and
background overview of the employed programs complemented by the
application of the tools to provided data sets. The third part of this
course will work through a typical use-case of downstream data
processing of shotgun lipidomics data following MS acquisition with
LipidXplorer. We will inspect, check and normalize the data as well as
calculate absolute quantities using internal class-specific standards
with lxPostman. We will then perform a qualitative and quantitative comparison of the
lipidomes using LipidSpace.
Learning goals:
Participants will be able to understand and explain the targeted shotgun MS and LC-MS workflows for lipidomics. They will learn the
fundamentals of the software tools used and how to choose parameters for
them. They will learn to understand and interpret the results of each
step of the pipeline.
Prerequisites:
Basic knowledge of lipidomics, analytical workflows in lipidomics and
basic familiarity with web-based and desktop applications. Please note
that you may need to install software on your computer to fully
participate in all exercises which may require the proper rights. The
workshop will be a mix of small lecture segments and hands-on exercises.
The trainers will be available for questions and assistance during the
workshop. We will use Zoom to host the workshop. To participate, please
ensure that the Zoom client software is installed on your computer.
Keywords:
Lipidomics, Shotgun, Targeted, LC-MS
Tools:
PeakStrainer and LipidXplorer, LipidCreator, Skyline, Goslin, lxPostman, LipidSpace
