https://lifs-tools.org/wiki/index.php?action=history&feed=atom&title=LX_Reference_Herzog_2012 2026-04-17T20:15:47Z Revision history for this page on the wiki MediaWiki 1.35.1 https://lifs-tools.org/wiki/index.php?title=LX_Reference_Herzog_2012&diff=37&oldid=prev 2018-02-28T14:28:35Z

<p>1 revision imported</p> <p><b>New page</b></p><div>====Queries, spectra and results====<br /> <br /> All data from the experiments can be found here: [https://wiki.mpi-cbg.de/wiki/lipidx/images/3/3f/Mass_Spectra_used_in_the_article.zip Mass_Spectra_used_in_the_article.zip]<br /> <br /> ====Materials and Methods====<br /> <br /> Please have a look into the article for a detailed description of the experiments: [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0029851 PLoS One 7(1): e29851]<br /> <br /> ====MFQL queries====<br /> <br /> For the acquisition in &#039;&#039;&#039;negative mode&#039;&#039;&#039; of samples with QStar with data-dependent acquisition (DDA), LTQ Orbitrap Velos with DDA mode and TSQ Vantage with precursor ion scan (PIS) mode (see Figure 3 of the article):<br /> <br /> * [[media:PE_negative_FAS.mfql | PE_negative_FAS.mfql]]<br /> * [[media:PG_negative_FAS.mfql | PG_negative_FAS.mfql]]<br /> <br /> For the acquisition in &#039;&#039;&#039;positive mode&#039;&#039;&#039; of samples with TSQ Vantage with neutral loss scan (PIS) mode (see Figure 4 of the article) for the loss of head groups of PE and PG: Δ m/z 141.02 for [M+H]+ molecular ions of PE and Δ m/z 189.04 for ammonium adducts [M+NH4]+ of PG. Because the actual spectra are stored as precursor ion scan spectra, the queries are customized to PIS:<br /> <br /> * [[media:PE_positive_NLS-_-PIS.mfql | PE_positive_NLS-_-PIS.mfql]]<br /> * [[media:PG_positive_NLS-_-PIS.mfql | PG_positive_NLS-_-PIS.mfql]]</div>

Nils.hoffmann